February 9, 2017 – Rising Tide Conservation, in partnership with the University of Florida Indian River Research and Education Center, is thrilled to announce the aquaculture of the Reef Butterflyfish (Chaetodon sedentarius)! This success is the first of 2017 for Rising Tide Conservation and Dr. Cortney Ohs and his fabulous team, including researchers Isaac Lee, Jason Broach, and Andrew Palau. Dr. Ohs writes the following about the history, procedure, and specifications for this fantastic accomplishment:

“We have one pair of Reef Butterflyfish (Chaetodon sedentarius) broodstock inside an 1800 liter round tank. They were acquired in May of 2015 from Marathon, FL, and were originally collected by Dynasty Marine. They began spawning three months later in August. They are fed four times a day with an Otohime EP1 pellet, PE Mysis, mullet roe, and Larry’s Fertility Frenzy. They spawn once every 2-3 days and produce approximately 3000-5000 eggs per spawn with >90% viable eggs. For this run, 3000 eggs were stocked out into a 440L fiberglass tank with black sides and a black bottom. The larval system was equipped with a 80 W UV sterilizer, two bag filters with 50 and 10 μm felt bags, a protein skimmer and trickle filter. One direct LED light was set on a 12 L: 12 D cycle and an ambient fluorescent light from an indirect source was on a 24 L: 0 D cycle. The tank was overturned 6x per day. Larvae were fed Parvocalanus crassirostris copepod nauplii (5.3/mL) twice a day and Brachionis plicatilis rotifers enriched with Selco S.presso (8.6/mL) once a day beginning 4 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 1 day post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 4 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 7 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 11 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 14 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae 17 days post hatch.

T-Isochrysis was added twice a day and timed with feedings of copepod nauplii. At 20 dph, we incrementally began adding newly hatched Artemia nauplii to the diet twice a day starting at 0.01 /mL and reaching 0.2/mL over the course of 8 days.

Reef butterflyfish (Chaetodon sedentarius) larvae 20 days post hatch.

Reef butterflyfish (Chaetodon sedentarius) larvae at 24 days post hatch.

Reef Butterflyfish (Chaetodon sedentarius) larvae at 26 days post hatch.

Reef Butterflyfish (Chaetodon sedentarius) larvae at 28 days post hatch.

At 28 dph, we stopped feeding rotifers. At 34 dph, we stopped feeding copepod nauplii.

Reef Butterflyfish (Chaetodon sedentarius) larvae at 38 days post hatch.

We began feeding Otohime A1 dry diet at 43 dph. The last mortality was recorded at 36 dph. Survival for this trial was 0.1% for three individuals. The larvae remained a bland grayish coloration and then begin developing a black band that goes vertically across the eyes. The posterior edges of the dorsal and anal fins began to darken up. Then they developed a yellow ridge across the top of the dorsal fins. The body then began to turn a brighter white.”

Juvenile Reef Butterflyfish (Chaetodon sedentarius) at 108 days post hatch.

This photo of the juvenile Reef Butterflyfish is at 108 days post hatch. The fish begin to take on the adult coloration at about 70 days old. Photograph opportunities were limited from 70-108 days old, but we look forward to replicating the success and getting more pictures! We hope that you’ll continue to follow our successes and support Rising Tide Conservation!

 

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